JB-4It is a unique polymer embedding material with higher morphological details than tissue embedded in paraffin.JB-4It is a water-soluble medium that generally does not require dehydration to anhydrous ethanol (except for high-density, bloody, or adipose tissue specimens).JB-4Suitable for routine staining, special staining, and histological staining, without the need for cleaning agents such as xylene and chloroform.
The polymerization of JB-4 resin is exothermic, and it can polymerize on ice or4-15The cooling of the temperature is easy to control,allJB-4 PlusAll types of packages must be used under a chemical smoke hood.JB-4Plastic slices can be cut0.5to3.0Microns or thicker, it is recommended to use glass knives, tungsten carbide steel knives, or those with the best resultsDiaTomeDiamond knife. Slices can be subjected to routine histological or histological staining. Attention: Immunohistochemistry is not recommendedJB-4Because methyl methacrylate cannot be removed from the slices and may block most of the antigen sites involved in antibody reactions. As an alternative, we recommendLR whiteLondon white glue.
JB-4 Plus provides our standardJB-4All the benefits and additional advantages of the formula: the addition of a special initiator reduces the heat generated during polymerization (the polymerization process is relatively mild), which is beneficial for temperature sensitive samples; The generated embedding block is transparent, and a clear polymer embedding matrix is essential in many histological and histological processes, especially in ophthalmic applications.
characteristic:
1. Slicing(0.5-2.0um)Can maintain good morphological structure
2. Room temperature90Transparent sample blocks can be formed in minutes
3. Maintaining good lipid and enzyme activity(4At low temperatures)
Compared to paraffin sections, it has higher transparency
5. For calcified bones and soft embryonic tissue embedding,JB-4Resin operation is also very simple
Product selection:
Item number |
product name |
specifications |
14270-00 |
JB-4 embedding reagent kit:800ml Solution A; 30ml Solution B; 12g Catalyst. |
cover |
14272-00 |
JB-4 Plus embedding kit:500 ml Solution A;15 ml Solution B;8 g Benzoyl Peroxide Catalyst |
cover |
Remarks:JB-4,JB-4+Anaerobic environment is required for embedding. It is recommended to use plastic embedding molds made of gelatin capsules or polystyrene materials (polystyrene embedding molds). For details, please search for the item number70177or70110wait.